Recruiter :
Darren Nelson
- Lab Manager position in cancer biology research lab
- Responsible for lab management, training, safety
- Experience in DNA repair, PARP, NAD+ metabolism preferred
The Laboratory Manager is responsible for lab management, lab operations, lab member training, lab safety, and lab maintenance. Further, this position is responsible for coordinating translational science collaborations and developing and analyzing patient and donor-derived tissue samples and cell cultures via biochemical, immunological, and functional analyses. Finally, this position is responsible for managing databases for Lab Equipment, DNA stocks, RNA stocks, and human/mouse cell stocks in support of a large cancer biology research lab.
The lab focuses on mechanisms of DNA repair, PARP, and NAD+ metabolism in human cells and the convergent role of these pathways in response to environmental genotoxins and chemotherapy. A major goal in the lab is to use biochemical, genetic, and imaging modalities to study DNA repair protein complexes that respond to DNA damage induced by environmental genotoxins and chemotherapy and how this pathway affects the regulation of cellular metabolism via ADP-ribosylation signaling and alterations in NAD+ metabolism. Recent efforts have focused on the role of DNA repair pathways and the regulation of PARP1/PARP2 activation in response to replication stress, the identification of novel targets to overcome PARP-inhibitor resistance and the development of novel biological tools and assays to evaluate genotoxic stress and PARP-activation that may be used for the discovery of novel compounds that may be used in the treatment of cancer and evaluating genotoxic response.
Techniques used in the lab include the genetic manipulation of cells in culture, genetic and epigenetic analysis of tumor cells, development and phenotypic characterization of transgenic cells, development and characterization of transgenic mice, RNA interference, CRISPR/cas9 gene editing, lentivirus production and viral transduction of mouse and human cells, molecular cloning of mouse and human cDNAs, DNA damage and repair assays, cell culture, cell cytotoxicity assays, and PCR.
Education and Experience
- Bachelor’s degree in Molecular Biology, Biology, or a related field is required.
- Master’s degree preferred.
- At least 5-7 years of experience working in organized research, or an equivalent combination of education and experience is required.
- 2 years of experience in bench research is preferred.
- Experience in culturing human primary cells and the development of primary cell lines from human tumors is preferred.
- Experience in immunoblotting, immunohistochemistry, and qRT-PCR preferred.
BACKGROUND AND EDUCATION CHECKS REQUIRED
Job Competencies
- High level of discretion to prioritize workflow and experimental methodologies.
- High-level communication skills with all levels of laboratory staff and collaborators.
- Excellent computer skills including databases and spreadsheets.
- High level of initiative and ability to work independently as well as collaboratively with a diverse group of people, with vastly different skills and training.
- Working some weekends and evenings may be required based on laboratory/experiment needs.
- Possesses willingness and ability to support a diverse and inclusive campus community.
